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2006•Chinese Heart JournalRequires access

Effect of estrogen on proliferation and cell cycle of cultured rabbit vascular smooth muscle cells induced by platelet-derived growth factor

Yonglan Zhou

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Abstract

AIM To investigate the effect of 17β estradiol(E_2) on the proliferation of vascular smooth muscle cells(VSMC) and the possible underlying mechanisms.METHODS A cell proliferating model of rabbit aorta VSMC induced by PDGF-BB was established.The proliferation was determined with MTT metabolism in subcultured VSMC exposed to different concentrations(10 and 100 nmol/L) of 17β estradiol and the cell cycle was examined by flow cytometry.RESULTS MTT metabolism of VSMCs induced by PDGF-BB was inhibited by E_2 and the cells numbers of G0/G1 phase increased and the cells numbers of G2/S phase decreased markedly(P0.01).CONCLUSION 17β estradiol inhibits the proliferation of VSMC induced by PDGF-BB,at least in part by preventing VSMCs entering S phase from G0/G1 phase.

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What this paper is about

AIM To investigate the effect of 17β estradiol(E_2) on the proliferation of vascular smooth muscle cells(VSMC) and the possible underlying mechanisms.METHODS A cell proliferating model of rabbit aorta VSMC induced by PDGF-BB was established.The proliferation was determined with MTT metabolism in subcultured VSMC exposed to different concentrations(10 and 100 nmol/L) of 17β estradiol and the cell cycle was examined by flow cytometry.RESULTS MTT metabolism of VSMCs induced by PDGF-BB was inhibited by E_2 and the cells numbers of G0/G1 phase increased and the cells numbers of G2/S phase decreased markedly(P0.01).CONCLUSION 17β estradiol inhibits the proliferation of VSMC induced by PDGF-BB,at least in part by preventing VSMCs entering S phase from G0/G1 phase.

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Available abstract

AIM To investigate the effect of 17β estradiol(E_2) on the proliferation of vascular smooth muscle cells(VSMC) and the possible underlying mechanisms.METHODS A cell proliferating model of rabbit aorta VSMC induced by PDGF-BB was established.The proliferation was determined with MTT metabolism in subcultured VSMC exposed to different concentrations(10 and 100 nmol/L) of 17β estradiol and the cell cycle was examined by flow cytometry.RESULTS MTT metabolism of VSMCs induced by PDGF-BB was inhibited by E_2 and the cells numbers of G0/G1 phase increased and the cells numbers of G2/S phase decreased markedly(P0.01).CONCLUSION 17β estradiol inhibits the proliferation of VSMC induced by PDGF-BB,at least in part by preventing VSMCs entering S phase from G0/G1 phase.

Key concepts: Vascular smooth muscle, Platelet-derived growth factor receptor, Cell growth, Cell cycle, Platelet-derived growth factor, Growth factor, Flow cytometry, Estrogen

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Effect of estrogen on proliferation and cell cycle of cultured rabbit vascular smooth muscle cells induced by platelet-derived growth factor — Research Paper | ScholarLens