2006•Unpublished venueRequires access
The expression of connective tissue growth factor and vascular endothelial growth factor in remnant rat kidney during the early process of compensatory renal growth following unilateral nephrectomy
Ding Guo-hu
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Abstract
Objective To study the role of connective tissue growth factor(CTGF) and vascular endothelial growth factor(VEGF) in the early stage of compensatory renal growth,and to explore the mechanisms of renal compensatory hypertrophy on rat kidney following unilateral nephrectomy.Methods The 48 Sprague Dawley rats were randomly divided into 2 groups with 24 rats in each.The rats in the operation group were underwent right nephrectomy operation and those of the control group were without any treatment.Six rats of each group were sacrificed on the 1st,4th,8th and 16th day of the experiment,respectively.The immunohistochemical method was used to investigate the expressions of CTGF and VEGF in the remnant left kidney of rats in each group.Results In the operation group,the weight of left kidney was increased obviously and the left kidney on the 16th day was obviously heavier than that on the 1st day of the experiment(P0.01).In the operation group,the expression of CTGF and VEGF protein,the glomerular area and volume were increased on the 16th day of the experiment compared with those of the 1st day(all P0.05).Furthermore,the indexes mentioned above in the operation group were much higher than those of the control group in the same time of the experiment(all P0.05).Conclusion The compensatory hypertrophy occurs in the remnant kidney of rats following unilateral nephrectomy.In addition,the expressions of CTGF and VEGF are increased in the early compensatory state of the remnant kidney.