Effect of total flavone of Litchi Chinensis Sonn(TFL)on tumor necrosis factor-related apoptosis-inducing ligand(TRAIL)in rat model of hepatic fibrosis

[Objective]To study the relationship between total flavone of Litchi Chinensis Sonn(TFL)and tumor necrosis factor-related apoptosis-inducing ligand(TRAIL)in rat model of liver fibrosis induced by bile duct occlusion and elucidate the role and mechanism of TFL on liver fibrosis.[Methods]Sixty male SD rats were randomly divided into three goups,sham operation,model and TFL treatment group.The hepatic fibrosis rat model was established by bile duct occlusion.TFL with a dose of 200mg/kg·d was intragastrically administrated to treatment group.Four weeks later,animals were sacrificed.Serum TRAIL levels were measured by enzyme-linked immunosorbent assay(ELISA).Serum levels of Hyaluronic acid(HA),Laminin(LN)and procollagen type Ⅲ(PC Ⅲ)were tested through radioimmunoassay(RIA).Liver tissue slides were stained by routine HE and Masson collagen stain to assess the degree of rat liver fibrosis.Expression of TRAIL in liver tissue was measured by immunohistochemical method called Strept-Avidin-Biotin enzyme Complex(SABC).[Results]Compared with model group,the liver tissue of TFL treatment group showed significant improvement in the degree of liver fibrosis.The serum TRAIL levels in the TFL treatment group and sham operation group were significantly lower than the model group(P0.05),and there was no significant difference(P0.05)between the former two groups.Serum TRAIL levels correlated with the expression of TRAIL and has a positive correlation significantly with the levels of HA,LN and PC Ⅲ in serum.[Conclusion]TRAIL promoted the development of liver fibrosis induced by bile duct ligation in rats,while TFL could ameliorate the degree of liver fibrosis.The mechanism may be related to inhibiting the expression of TRAIL in the liver tissues.