Gene cloning and expression of hybrid HPV6b L1/16E7 protein in insect cells

Objective In order to make the genetic engineering vaccine against CA and cervical cancer, the hybrid HPV6b L1/16E7 protein was expressed in Sf 9 insect cells with baculovirus express vector system. Methods The gene fragment of hybrid HPV6b L1/16E7 has been amplified by PCR and cloned into the baculovirus transfer vector pVL1393, a recombinant baculovirus expression vector was constructed to express the hybrid HPV6b L1/16E7 gene in insect cells. Results The hybrid HPV6b L1/16E7 protein was expressed in Sf 9 insect cells, assembled into virus like particles (VLPs). Conclusion The hybrid HPV6b L1/16E7 VLPs expressed in Sf 9 insect cells is quite potential in the future research, mechanism of HPV infection, for the development of genetic engineering vaccine.