Study of loss of heterozygosity (LOH) on multiple chromosome arms in hepatocellular carcinomas

Objectives:Study of loss of heterozygosity (LOH) at 38 loci on five chromosomes in 38 cases of hepatocellular carcinoma,and the possible relations betwee the LOH and clinicopathologic changes and hepato viral infection of the HCC.Methods:PCR based microsatellite polymorphism analysis technique was used to detect LOH in HCC.Results:High frequency of LOH was detected on chromosome 1p36 concentrated at locus D1S243 (51 6%)and 1p31 at locus D1S186 (48 1%). On 9p24 at locus D9S54 (61 8%) and 9p21 concentrated at loci D9S1747 (52 4%) and D9S1752 (51 8%),on chromosome 10, only locus D10S1223 (53 3%)on 10q23～24 showed high frequency of LOH. All 5 loci detected on chromosome 16 showed high frequency of LOH more than 50%,with the highest frequency of LOH at locus D16S413 (70 8%) on 16q 24. On chromosome 17, high frequency of LOH concentrated on 17p13 at TP53 gene locus (53 8%)and locus D17S520 (56 8%). HBV and HCV coinfection in HCC is associated with LOH at TP53 locus and p15 gene locus.Conclusions:The results indicated that high frequency of LOH on multiple chromosome arms were involved in hepatocarcinogenesis,there may be more than one putative TSG harbored in these chromosome arms associated with HCC.Coinfection of HBV and HCV is closely associated with LOH at p53 and p15 gene loci.