Expression of C-fos in the cortex and hippocampus of rats after Pilocarpine-induced seizure

Objective: To observe the expression of C-fos protein in the brain at different stages after pilocarpine-induced seizure.Methods: Epileptic seizure was induced by pilocarpine in rats.The behavioral changes were observed,and the expression of C-fos in the brain in different stages of seizure was detected by immunohistochemistry.Results: The C-fos expression in hippocampal CA1,CA3,dentate gyrus and cerebral cortex was significantly different at different stages of seizure(P0.05).In the early stages of seizure(stage I and II),C-fos was mainly expressed in the cortex.With increasing of the degree of convulsion,C-fos expression expanded in the dentate gyrus,CA1,CA3 and cortex in stages IV and V.Conclusion: In the early stages of epileptic seizure,C-fos begins to express,so in order to decrease neurons damage measurements should be taken to early monitor.