Lectin-like oxidized low-density lipoprotein receptor 1 mediates the effect of Bezafibrate on expression of PAI-1 induced by ox-LDL in HUVECs

Objective To investigate how Bezafibrate impact on secretion of plasminogen activator inhibitor-1 induced by ox-LDL in human umbilical endothelial cells(HUVECs), and how Lectin-like oxidized low-density lipoprotein receptor 1(LOX-1) act in the course.MethodsHUVECs were incubated in vitro.Expression of PAI-1 mRNA were determined by RT-PCR, protein of PAI-1 were examined by ELISA.ResultsIncubation of HUVECs with 50 μg/mL ox-LDL enhanced the expressions of PAI-1, compared with the normal group(P 0.01).The expression of PAI-1 induced by ox-LDL were reduced in HUVECs pretreated with 250 μg/mL poly(Ⅰ) for 2 h and then incubated with 50 μg/mL ox-LDL for 24 h.Incubation of HUVECs with different concentration of Benzafibrate(50, 100, 200 μmol/L) decreased the expressions of PAI-1(P 0.01).The expression of PAI-1 induced by ox-LDL were reduced in HUVECs pretreated 250 μg/mL poly(Ⅰ) for 2 h and then incubated with 100 μmol/L Bezafibrate for 2 h, and then 50 μg/mL ox-LDL for 24 h(P 0.01).ConclusionOx-LDL may up regulate the expression of PAI-1, and LOX-1 blocker may partly inhibit this up regulation.The results suggest that the expression of PAI-1 induced by ox-LDL in HUVECs is mediated by the action of LOX-1.Different concentration benzaf-ibrate may decrease the expression of PAI-1, which were mediated by the action of LOX-1, then impact the fibrinolytic system, and play an important role in the development of arteriosclerosis.