The anti-calcification effect of basic fibroblast growth factoron canine saphenous vein interstitial cells in vitro

Objective To observe the anti-calcification effect of basic fibroblast growth factor (bFGF) on canine saphenous vein interstitial ceils (SVICs) in vitro.Methods Canine SVICs were cul- tured arid derided into control group,calcific group and bFGF group.After 2 weeks,cellular layer was stained by yon kossa.Calcium deposition and alkaline phospatase (ALP) activity of cellular layer,osteocal- cin (OC) in cultured supernatant were analyzed.Results (1) After 2 weeks,in calcific group,calcific nodules were enriched by yon kossa staining.In control group,there were cellular nodules but calcification was mildly positive.In bFGF group,there was no cellular nodules and calcification was neganve by von kossa staining;(2) Calcium deposition in calcific group was 27 times higher than that of control group [(5.384±0.443) vs (0.192±0.024),P＜0.01].The activity of ALP in the calcific group was 2.13 times higher than of control group [(9.611±0.639) vs (3.069±0.178),P＜0.01].Both calcium and ALP activity were very lower in bFGF group [(2.198±0.173) vs (5.384±0.443),P＜0.01; (4.819±0.334) vs (9.611±0.639),P＜0.01].OC of sup ernatanz was increased at 6th day,reached the peak at 12th day,and then slightly decreased in calcific group.But in bFGF and control groups the content of OC was maintained at a lower level.Conclusion The cultured canine SVICs have osteoblast phenotype and are calcified in calcified medium in vitro,bFGF can inhibit the calcification significantly.