Role of NF-Kappa B Signal Pathway in the Expression of AQP5 During Lung Ischemia Reperfusion Injury Under Deep Hypothermia in Rats

Objective:To study the expression of Aquaporin 5(AQP5)during lung ischemic reperfusion injury after deep hypothermia in rats and the regulatory effects of NF-κB signal pathway. Methods:Thirty Wistar rats were randomly divided into sham operation group(sham group),ischemia reperfusion after deep hypothermia group(I/R group),and PDTC intervention group(I/ R + PDTC group).The left lower hilus pulmonis of rats in I/R group were blocked for 30min after body temperature cooled to deep hypothermia and soon opened and rewarmed.Rats in sham group only underwent thoracotomy without cooling down and blocking.PDTC were injected intraperitoneally 2days before the experiment in rats of PDTC group while other manipulation was the same to I/R group.The lung specimens were harvested after rewarming for 1hfor pathological analysis,and the wet/dry(W/D)weight ratio were also measured.Real-time PCR and Western-blot were conducted to detect the expression of AQP5and NF-κB p65.Results:Compared with sham group,the expression of AQP5in I/R group reduced(P0.01)and expression of NF-κB p65increased(P0.01),and W/D ratio increased(P0.01),and the structure of lung tissue was seriously impaired;compared with I/R group,the expression of AQP5in PDTC group increased(P0.01)and expression of NF-κB p65reduced(P0.01),W/D ratio reduced(P 0.01),and the destruction of lung tissue was more severe.Compared with sham group,expression of AQP5in PDTC group decreased,while expression of NF-κB p65increased(P0.01), and W/D ratio increased(P0.01).Conclusion:The increased expression of AQP5and reduction of NF-κB p65may correlated with acute lung injury caused by deep hypothermic ischemia reperfusion,and it suggests NF-κB signal pathway might be involved in the regulation of AQP5.