Resuscitation for hemorrhagic shock rats:lactated Ringer's solution with whole blood or red blood cells

Objective To compare the resuscitation effect of lactated Ringer's solution(LR) combined with hydroxyethyl starch(HES),whole blood or red blood cells on hemorrhagic shock rats.Methods SD rats(n=24) were inflicted with a sharp resection of 75% of the tail to initiate the hemorrhage.When the blood pressure(BP) was decreased to 40 mmHg,LR solution was infused to the rats for 1 h.Then the animals were randomly divided into 3 groups,LR+HES(group Ⅰ),LR+HES+auto-whole blood(group Ⅱ),and LR+HES+auto-red blood cells groups(group Ⅲ),and received the corresponding infusion respectively for 2 h when the BP was increased to 80 mmHg.The blood samples were collected before shock,1 h after shock and 2 h after resuscitation.Hematologic and hemodynamics parameters,such as blood cell count,heamoglobin,hematocrit,platelet count,heart rate,BP,survival time,left intraventricular systolic pressure(LVSP),maximal change rate of left intraventricular pressure(±dp/dtmax),were measured and recorded.Results Hematologic parameters of each group had significant difference in 1 h after shock compared with 2 h after resuscitation,and the 2 time points compared with before shock.Those of group Ⅱ and group Ⅲ were significantly higher than those of group Ⅰ.In every group,the parameters at the time of 2 h after resuscitation had significant difference to those at 1 h after shock.Group Ⅱ and group Ⅲ had a significantly longer survival time compared with group Ⅰ.HR,LVSP and ±dp/dtmax in each group at 1 h after shock and 2 h after resuscitation were significantly decreased compared with before shock.Those of group Ⅱ and group Ⅲ were significantly higher compared with group Ⅰ;those of each group in 2 h after resuscitation were markedly increased compared with 1 h after shock.Conclusion LR solution plus HES has efficient effect on the resuscitation of hemorrhagic shock rats to maintain normovolemia,and the infusion of auto-red blood cells is helpful to correct anemia.Plasma-poor red cell concentrates is safe and causes no abnormal phenomena of blood clotting.