EFFECTS OF GENISTEIN ON GROWTH OF MASTOPATHIA CELL MCF-7

Objective To study the effects of genistein on growth inhibition,the cell cycle blocking,cell apoptosis induction of mastopathia cell MCF-7.Methods MCF-7 cells were normally cultured,then during the cell logarithm growth period,the cells were treated with genistein of different doses.The growth of mastopathia cell MCF-7 was observed by MTT assay,flow cytometer,and giemsa staining.Results ① MTT assay showed that the absorptions(indicating the inhibition on cellular growth) of 20,40,80,160 μmol·L~(-1) of genistein were 0.460±0.018,0.450±0.020,0.440±0.020,0.385±0.040 respectively on the lst day of cell culture,which were lower than that of control(0.520±0.023,P0.05).The changes on the 2nd,3rd,4th day were similar to the lst day.②The cell cycle G_2% of 80,160 μmol·L~(-1) of genistein(50.0%,57.5% respectively) were higher than that of control(6.8%).③The apoptotic rates of 10,80 μmol·L~(-1) of genistein were 6.51%,20.03% respectively while that of control was 0.50%.Conclusion Genistein can obviously suppress the cell growth,block the cell cycle(especially in G_2 period),and induce the cell apoptosis.