The role of heat shock protein 27 in acute liver failure induced by pretreatment with lipopolysaccharide

Objective To investigate the role of heat shock protein 27(HSP27) in acute liver failure induced by pretreatment with lipopolysaccharide.Methods Male C57BL/6 mice were randomly divided into 5 groups:control group(with no treatment);liver injury group,which were injected intraperitonelly with D-Galn(700 mg/kg) and LPS(10μg/kg) dissolved in 1 mL sterile saline;LPS pretreatment group,which were injected with LPS(10μg/kg)24 hours before D-Galn/LPS treatment;HSP27 interference group,murine HSP27 was interfered by short hairpin RNA and mice were subjected to D-Galn/LPS with LPS pretreatment;Negative control group,which were also injected with vector virus and treated in the same way with HSP27 siRNA group.Serum ALT and AST levels,hepatic histological damages and mRNA levels of pro-inflammatory cytolines(TNF-αand IL-6) were measured.Results LPS pretreatment significantly reduced liver injury induced by D-Galn/LPS.The interference of HSP27 aggravated liver injury,which could be known from the higher levels of ALT and AST(P0.05),as well as the severer histological damage and more expression of TNF-αand IL-6(P0.05).Reducing the expression of HSP27 largely abrogated the protective effects induced by LPS pretreatment.Conclusion LPS pretreatment could protect against acute liver failure through HSP27 dependent pathways.