Determination of chloramphenicol residues in acquatic products by ultra performance liquid chromatography-electrospray tandem mass spectrometry

An ultra performance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) method was developed to determine the residue of chloramphenicol in aquatic products.The samples,labled by the isotopically internal standard,were extracted by ethyl acetate,then defatted with n-hexane(or purified by SPE).Chromatographic separation was achieved by using UPLC,and the detection was performed by mass spectrometry.Results showed that the recoveries for chloramphenicol at the spiked concentrations of 0.05～1.0 μg/kg ranged from 84.9% to 103.3% and the relative standard deviations (RSDs) for the determination of chlommphenicol were 3.2%～5.2%.The limit of quantity (LOQ) was 0.02 μg/kg.The method is rapid and sensitive,suitable for the determination of the chloramphenicol residue in acquatic products.