Effect of E2F decoy oligodeoxynucleotide on intimal hyperplasia in rat vein graft

Objective To study the effect of E2F decoy oligodeoxynucleotide (ODN) on intimal hyperplasia in auto vein graft of rat.Methods Auto vein graft models were developed in 30 Wistar rats.The pluronic gel containing E2F decoy double-strand ODN was applied to the adventitia of transplanting veins.The veins were collected at 14 day after grafting.Paraffin-embedded veins were cut into sections.The intima thickness and expression of c-myc and proliferating cell nuclear antigen (PCNA) were determined using histological methods.Pluronic gel containing mismatch ODN or nothing served as control group.Results There was significant intimal hyperplasia in control vein grafts and obvious PCNA or c-myc expression in smooth muscle cells.Mismatch ODN had no effect on inhibition of expression of PCNA or c-myc and intimal thickening.In veins treated with 100 μg E2F decoy ODN,the positive rate of PCNA or c-myc was significantly reduced to (20.41±4.15)% or (10.83±2.87)%,decreased by 50% or 34% vs.control veins (P0.05).Intimal thickness was (18.2±2.8) μm and decreased by 44% vs.control group (P0.05) at the same time and the larger dose of ODN (200 μg) led to more obvious inhibition of expression of PCNA or c-myc and neointimal hyperplasia.Conclusion Treatment of vein graft with E2F decoy ODN decreased intimal thickening in a dose-dependant manner by inhibition of expression of c-myc and PCNA.