The influence of chemotherapy combined with Shenfu Injection on the T-lymphocyte subsets,IL-6,TNF-α of patients with acute leukemia

Objective:To investigate the influence of chemotherapy combined with Shenfu Injection on the T-lymphocyte subsets,interleukin-6(IL-6),tumor necrosis factor-α(TNF-α) of the patients with acute leukemia and the immune mechanisms in treating acute leukemia in further analysis.Methods:30 patients with acute leukemia treated with chemotherapy and Shenfu Injection in our hospital from January 2008 to January 2010 were taken as treatment group and 30 patients with acute leukemia in the same time given chemotherapy alone were taken as control group;the T-lymphocyte subsets(CD4+T,CD8+T-lymphocyte percentage and the ratio of CD4+T/CD8+T) of the two groups were determined by flow cytometry,and the levels of serum IL-6 and TNF-α were detected by ELISA.Results:The total effective rate of the treatment group was 76.7%,the total effective rate of the control group was 56.7%,the difference was statistically significant between two groups(P0.05).After treatment,the level of CD4+T,CD4+T/CD8+T of treatment group and control group [(31.30±6.25)%,(30.34±4.25)%,(1.08±0.16);(27.32±7.31)%,(29.25±6.18)%,(0.96±0.29)] were significantly higher than before treatment [(25.43±7.14)%,(32.76±5.60)%,(0.81±0.25);(26.37±6.46)%,(31.52±4.74)%,(0.78±0.16)],the treatment group improved more significantly than the control group(P0.05);after treatment,the level of IL-6 and TNF-α in control group and treatment group [(156.33±71.69) ng/L,(191.02±74.96) μg/L;(107.22±63.43) ng/L,(160.08±78.41) μg/L] were significantly lower than before treatment [(295.98±84.69) ng/L,(1138.91±147.89) μg/L;(307.97±82.52) ng/L,(1122.13±145.64) μg/L],the treatment group was decreased significantly than the control group(P0.05).Conclusion:Chemotherapy combined Shenfu Injection can significantly improve immune function in patients with acute leukemia and they have some regulatory role for the proliferation and differentiation of hematopoietic stem cells and leukemia cells.