Effect of Subcrustal Tissue Fluid on Endothelial Cell Monolayer Permeability Under Hydrostatic Perfusion

Objective To investigate the effect of subcrustal tissue fluid (STF) on the endothelial cell (EC). Methods The vascular permeability of perfused endothelial cell monolayer cultured on micropore filter membrane was studied to determinate the filtration coefficient (kf), transendothelial cell monolayer volume flux (Jv), and osmotic reflection coeficient (б) after EC was incubated with STF for 24 hours. Furthermore, the change of cell activity was determined and morphology was observed. Results Incubation of EC with 20% STF for 24 hours decreased the activity of human umbilical vein endothelial cell (HUVEC), shrinked HUVEC, enlarged intercellular space and increased permeability of endothelial cell monolayer. Conclusions STF can damage endothelial cells and induce increase of EC monolayer permeability.