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2012•Anhui Medical and Pharmaceutical JournalRequires access

Determination of beiberine hydrochloride in xiaokeling tablets by HPLC

Jiang Shu-fei

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Abstract

Objective To establish a method for determination of Beiberine hydrochloride in Xiaokeling tablets.Methods By HPLC method,the column was Century SIL C18(250 mm×4.6 mm,5 μm).Acetonitrile-0.05 mol·L-1 potassium dihydrogen phosphate solution(51∶ 49,each 1 000 ml of the mobile phase containing twelve sodium alkyl sulfonate 1.7 g) as mobile phase.The flow rate was 1 ml·min-1.Detecting wavelength was at 345 nm and column temperature at 30℃.The external standard-method was adopted for the assay.Results Berberine hydrochloride showed good linear relationship in the range of 0.038~0.304 μg and regression equation was A=4 281 910 C+3 319(r=0.999 9),with a mean recovery rate of 98.01%,RSD 1.16%.Conclusion The method is good in separable effect,reproducibility and sensitivity.

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Objective To establish a method for determination of Beiberine hydrochloride in Xiaokeling tablets.Methods By HPLC method,the column was Century SIL C18(250 mm×4.6 mm,5 μm).Acetonitrile-0.05 mol·L-1 potassium dihydrogen phosphate solution(51∶ 49,each 1 000 ml of the mobile phase containing twelve sodium alkyl sulfonate 1.7 g) as mobile phase.The flow rate was 1 ml·min-1.Detecting wavelength was at 345 nm and column temperature at 30℃.The external standard-method was adopted for the assay.Results Berberine hydrochloride showed good linear relationship in the range of 0.038~0.304 μg and regression equation was A=4 281 910 C+3 319(r=0.999 9),with a mean recovery rate of 98.01%,RSD 1.16%.Conclusion The method is good in separable effect,reproducibility and sensitivity.

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Available abstract

Objective To establish a method for determination of Beiberine hydrochloride in Xiaokeling tablets.Methods By HPLC method,the column was Century SIL C18(250 mm×4.6 mm,5 μm).Acetonitrile-0.05 mol·L-1 potassium dihydrogen phosphate solution(51∶ 49,each 1 000 ml of the mobile phase containing twelve sodium alkyl sulfonate 1.7 g) as mobile phase.The flow rate was 1 ml·min-1.Detecting wavelength was at 345 nm and column temperature at 30℃.The external standard-method was adopted for the assay.Results Berberine hydrochloride showed good linear relationship in the range of 0.038~0.304 μg and regression equation was A=4 281 910 C+3 319(r=0.999 9),with a mean recovery rate of 98.01%,RSD 1.16%.Conclusion The method is good in separable effect,reproducibility and sensitivity.

Key concepts: Chromatography, Berberine hydrochloride, Chemistry, Potassium, High-performance liquid chromatography, Reproducibility, Sodium, Acetonitrile

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