Multiresidue Determination of Ten Sulfonamides in Honey by Solid-phase Extraction and High Performance Liquid Chromatography

A multiresidue analytical method was developed for the simultaneous determination of ten sulfonˉamides in honey using solid-phase extraction and high performance liquid chromatography.The whole process includes blenderextraction of10.00gsamples withchloroform,evaporationto dryness,partition with phosphoˉrous acid buffer solvent,sample clean-up through a C 18 solid-phase extraction column with acetonitrile,and reversed-phase high performance liquid chromatography with a UV detector.The detection wavelength was270nm;the column temperature was45℃;and the mobile phase for gradient elution was a mixture of aqueˉous phosphorous acid-acetonitrile.The recoveries of the added10sulfonamides at levels of0.010～1.0mg/kg were found to be in the range of86.5%～100.8%with coefficients of variation of3.5%～8.1%.The linear range was0.010～10μg/mL,and the detection limit was0.005mg/kg.This method is simple,rapid,reproducible,sensitive and selective.