Multiplex RT-PCR detection for influenza A virus

We aimed to developed a rapid,sensitive and specific multiplex RT-PCR,which could detect influenza A H1N1 influenza virus,seasonal H1N1 influenza virus,and seasonal H3N2 influenza viruses simultaneously.A new RT-PCR detection with multiplex primers was set up,which was applied to influenza nucleic acid testing technology in the laboratory.In the experiment the three pairs of primer of sub-type virus about the influenza A virus were used in the same RT-PCR reaction system to detect the throat swab samples of suspected influenza.As result,the multiplex RT-PCR on the sub-types virus of influenza A virus had high sensitivity and specificity,and can directly detect the sub-types from suspected influenza specimens.The new multiplex RT-PCR had high specific and sensitive like common RT-PCR,but it was more rapid and convenient than conventional RT-PCR and virus isolation methods.