Wide Cross Breeding of Lily and Identification of Authenticity

Using five wild lily and two lily cultivars as parents.Designing six cross-combination for wide cross breeding.Distant hybrids was obtained successfully by field planting way and ovary culture finally.They were identified by morphology and RAPD molecular identification.The results showed that crossing combination Lilium longiflorum×Lilium callosum obtaining three hybrid seedlings by ovary culture,Lilium callosum×Lilium regale,Lilium callosum×'Snow Queen',Lilium pumilum(Provenance of Heilongjiang)×'Snow Queen',Lilium pumilum(provenance of Heilongjiang)×'Siberia',Lilium pumilum(provenance of Chengde)×'Siberia' obtaining hybrid seeds.The seed set ratio were 27.0%,1.4%,31.4%,0.04% and 0.08% respectively.Plant type of hybrid seedlings are matroclinous plants and intermediate plants.The other indexs of morphology lies between parents or better than parents.RAPD molecular identification showed that the hybrids has not only additive banding,solo-parent banding,but also extraordinary banding which is the fusion of its parents,and new bandings appearing in the patterns.The hybrids are true hybrid and can be used to quality breeding of Lily.