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2012•Journal of Pathogen BiologyRequires access

A method for degenerate primer PCR extraction of genomic DNA from Pythium sp.GY1938

Minhui Li

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Abstract

Objective To explore the best method for degenerate primer PCR extraction of genomic DNA from the Pythium sp.GY1938 strain.Methods Five methods were used to extract genomic DNA and their efficiency was separately evaluated with specific and degenerate primer PCR.Results Results of specific primer PCR showed that there were no significant differences in the five methods.However,results of degenerate primer PCR showed that the benzyl chloride method was more effective because it a larger number of clearer bands.Conclusion The benzyl chloride method was best suited to degenerate primer PCR extraction of genomic DNA from the Pythium sp.GY1938 strain.

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What this paper is about

Objective To explore the best method for degenerate primer PCR extraction of genomic DNA from the Pythium sp.GY1938 strain.Methods Five methods were used to extract genomic DNA and their efficiency was separately evaluated with specific and degenerate primer PCR.Results Results of specific primer PCR showed that there were no significant differences in the five methods.However,results of degenerate primer PCR showed that the benzyl chloride method was more effective because it a larger number of clearer bands.Conclusion The benzyl chloride method was best suited to degenerate primer PCR extraction of genomic DNA from the Pythium sp.GY1938 strain.

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Available abstract

Objective To explore the best method for degenerate primer PCR extraction of genomic DNA from the Pythium sp.GY1938 strain.Methods Five methods were used to extract genomic DNA and their efficiency was separately evaluated with specific and degenerate primer PCR.Results Results of specific primer PCR showed that there were no significant differences in the five methods.However,results of degenerate primer PCR showed that the benzyl chloride method was more effective because it a larger number of clearer bands.Conclusion The benzyl chloride method was best suited to degenerate primer PCR extraction of genomic DNA from the Pythium sp.GY1938 strain.

Key concepts: Primer (cosmetics), genomic DNA, Biology, Polymerase chain reaction, DNA extraction, DNA, Primer dimer, Strain (injury)

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