Effect of Adenovirus-mediated IL-24 Gene on Inhibition of A549 Cell Growth

Objective To study the effect of recombinant adenovirus expressing IL-24 on A459 cells.Methods The IL-24 gene was transfected into human lung carcinoma cell line A549 with a replication-incompetent adenovirus vector.The mRNA expression of IL-24 was detected by RT-PCR.The morphological changes of A549 cells before and after the infection of Ad-hIL-24 were observed by using light microscope and fluorescence microscope.The inhibition of cell growth and the apoptotic effect were tested by MTT essay and flow cytometry.Caspase-3 was explored by means of immunocytochemistry.Results IL-24 was proved to be successfully transcribed in A549 cells.The growth of A549 cells was suppressed obviously after transfection of Ad-hIL-24.The apoptotic rate of A549 cells was significantly elevated.IL-24 could up-regulate the expression of caspase-3.Conclusion Recombinant adenovirus mediated IL-24 expression can induce growth suppression and apoptosis in A549 cells.