Combined detection of EGFR and HER2 in gastric adenocarcinoma by CISH and IHC

Objective Tocomparetheexpressionsofepidermalgrowthfactor(EGFR)andhumanepidermalgrowth factor receptor 2(HER2) in gastric adenocarcinoma by chromogenic in situ hybridization(CISH) and immunohistochemistry(IHC),respectively.Methods EGFR and HER2 expressions of 85 cases with gastric adenocarcinomas were detected by CISH and IHC.Results Among 29 patients with positive EGFR,four cases with(3+) showed gene amplification;eight of 11cases with(2+) showed gene amplification;only one of the 14 cases with(1+) showed gene amplification.Among 13 patients with positive HER2,four cases with(3+) showed gene amplification;five of six cases with(2+) showed gene amplification;three cases with(1+) failed to show gene amplification.There was no correlation between gene amplification and EGFR or HER2.Conclusions Cases with(3+) of IHC is consistency with CISH preferably,and CISH has a high specificity in predicting gene status.