Effect of NAC on iNOS,GSH and GSH-PX in hepatic tissue of mice with schistosomiasis japonicum

Objective: To study the effect of N-acetylcysteine(NAC) on iNAS,NO,GSH and GSH-PX in hepatic tissue of mice with schistosomiasis japonicum.Methods: Mice were randomly divided into control group,model group,prevention group a,prevention group b,treatment group a and treatment group b.Gastric lavage with 200 mg/kg and 400 mg/kg NAC,twice per day were carried out to mice in prevention group a and prevention group b as infection,and mice in treatment group a and treatment group b 6 weeks after infection.Mice were scarified on the 42nd day in control group and model group,the 56th day in prevention group and treatment group.The serum and liver were collected.The indexes about hepatic pathological changes in serum and liver tissues were detected,including inducible nitric oxide synthase(iNOS),nitric oxide(NO),glutathione peroxidase(GSH-PX),and reduced glutathione hormone(GSH).Results: The concentration of NO and the activity of GSH in hepatic tissue showed different change trend.The activity of iNOS and NO level in prevention group were between the results of control group and model group.The GSH level of prevention group a and treatment group a was significant lower than control group and model group(P0.05).The levels of iNOS,NO and GSH-PX were relative to dose of NAC,but there was no relationship between these indexes level and action duration of NAC,and interactivity of NAC action duration and dosage.While GSH level was associated with interactivity of NAC action duration and dosage,and NAC dosage.Conclusion: Serum levels of NO and GSH can′t be indices of content of NO and GSH in hepatic tissue.NAC can improve the NO content,and activity of iNOS and GSH-PX.That is possible mechanisms by which NAC at small dosage can alleviate hepatic damage due to Schistosoma japonicum.But there is critical value of NO,iNOS and GSH-PX.