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Production conditions and enzymatic properties of lipase from rhizopus ZZ-3

Wei Dong-zhi

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Abstract

In this paper the production conditions of lipase from Rhizopus ZZ-3 were optimized and its enzymatic properties were studied.The results indicated that the suitable conditions for lipase production from Rhizopus were as the follows: soy powder 3 g /100mL,(NH4)2S04 0.3 g /100mL,MgS04 0.2 g /100mL,K2HPO4 0.2 g /100mL,soybean 0.3 g /100mL,pH natural.The highest lipase activity was 124.60 U /mL on the condition of 28 ℃,150 r / min.The properties of the lipase was also characterized.The optimal temperature and pH of lipase was 37 ℃ and 7.5.The lipase was relatively stable in pH range of 7-9 and at 40 ℃.

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What this paper is about

In this paper the production conditions of lipase from Rhizopus ZZ-3 were optimized and its enzymatic properties were studied.The results indicated that the suitable conditions for lipase production from Rhizopus were as the follows: soy powder 3 g /100mL,(NH4)2S04 0.3 g /100mL,MgS04 0.2 g /100mL,K2HPO4 0.2 g /100mL,soybean 0.3 g /100mL,pH natural.The highest lipase activity was 124.60 U /mL on the condition of 28 ℃,150 r / min.The properties of the lipase was also characterized.The optimal temperature and pH of lipase was 37 ℃ and 7.5.The lipase was relatively stable in pH range of 7-9 and at 40 ℃.

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Available abstract

In this paper the production conditions of lipase from Rhizopus ZZ-3 were optimized and its enzymatic properties were studied.The results indicated that the suitable conditions for lipase production from Rhizopus were as the follows: soy powder 3 g /100mL,(NH4)2S04 0.3 g /100mL,MgS04 0.2 g /100mL,K2HPO4 0.2 g /100mL,soybean 0.3 g /100mL,pH natural.The highest lipase activity was 124.60 U /mL on the condition of 28 ℃,150 r / min.The properties of the lipase was also characterized.The optimal temperature and pH of lipase was 37 ℃ and 7.5.The lipase was relatively stable in pH range of 7-9 and at 40 ℃.

Key concepts: Lipase, Rhizopus, Chemistry, Enzyme, Food science, Biochemistry, Fermentation

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Production conditions and enzymatic properties of lipase from rhizopus ZZ-3 — Research Paper | ScholarLens