2011•Zhongguo shengwu gongcheng zazhiRequires access
Overexpression of Heat Shock Protein70 Enhances Production of Antibody in Chinese Hamster Ovary Cells
Yigang Tong
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Abstract
The production of recombinant antibody was significantly enhanced by the method of overexpression of Heat Shock Protein 70 in Chinese Hamster Ovary(CHO) cells.The gene HSP70 was cloned from CHO/dhfr-cells,then HSP70 expression plasmid pcDNA3.1-HSP70 was constructed and transfected into CHO/dhfr-cells.CHO cell line overexpressing HSP70 stably was isolated and the expression of HSP70 gene was analyzed through RT-PCR.Anti-HBs chimeric antibody expression plasmids were transfected into the HSP70 cell line,ELISA was performed to test the production of anti-HBs antibody.It demonstrated that the overexpression of HSP70 can enhance the production of recombinant antibody in CHO/dhfr-cells(P0.05).