Isolating Cultivation and Morphology of Nematode-destroying Fungi

The nematode-destroying fungus had an optimal growth on 0.4 g/L CMA.The liquid medium of same CMA can be used to preserve fungus for a longer time.In order to differentiate the nematode-destroying fungus from other fungi,the alive third larvae of nematode should be given for inducing the fungus to produce trapping organs(rings and networks) when it was isolated.The vegetative hypha and conidia were formed in different development stages.The colourless hypha of Arthrobotrys oligospora developed well in different length of segments and the single conidiophore surrounded by two or more circles of conidia,which look like plum blossoms,stretched up.The conidia are colourless,stout and inverse egg-shaped,and they are divided into two unequal parts:one with a rounder dissociative end is biger and another with a sharper end connected to the conidiophore is smaller.