Effects of penehyclidine hydrochloride on acute lung injury in rats

Objective To investigate the beneficial effect of penehyclidine hydrochloride in rats acute lung injury(ALI) induced by lipopolysaccharide(LPS).Methods LPS(5 mg/kg) was used to reproduce ALI model in rats.Forty adult SD rats were randomly divided into five groups: group NS with normal saline;ALI model group(group LPS);and PHCD was given respectively 1 h before LPS injection in low-dose group(0.03 mg/kg),middle –dose group(0.1 mg/kg) and high-dose group(0.3 mg/kg).At 4 h after LPS was given,the animals were killed.The pathological manifestation of lungs were observed,and the lung index and lung W/D ratio were calculated,and the intension of nuclear factor-κB,nitric oxide synthase(NOS) and myeloperoxidase(MPO) activity in the lung,the concentration of nitric oxide(NO) and malondialdehyde(MDA) in serum and lung were detected.Results Obvious ALI pathological manifestation in the lungs of LPS group rats were observed.Compared with NS group,the expression of NF-κB and NOS activity in lungs and concentration of NO in serum and lung increased significantly in LPS group(P0.01) as well as lung index and concentration of MDA and MPO activity in lung(P0.01).Compared with LPS group,the pathological manifestation in the lung was ameliorated and the expression of NF-κB and NOS activity in lung,the concentration of NO and MDA,and lung index decreased significantly in different doses of PHCD groups(P 0.01).Conclusion NF-κB and NOS-NO pathway were involved in the pathogenesis of ALI in rats model and PHCD could inhibits endotoxin induced NO release and lilpoperoxidaton.