2006•PubMedRequires access
[Construction of eukaryotic expression recombinant plasmid of trichomonas vaginalis ferredoxin gene].
Hui Xie, Yajing Wang, Chao-nan Tie, Shi-liang Bi, Pei-na Liu
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Abstract
Total DNA was extracted from T. vaginalis with Chelex-100 method and used as templates for PCR. The ferredoxin gene was directionally cloned into plasmid pMD-18T vector and subcloned into eukaryotic expression vector pcDNA3. 1 (+). The transformants were screened and identified by PCR and restriction analysis. The size of amplified ferredoxin gene was 306bp and the DNA sequence of cloned gene was same with that published.