2008•Journal of Yantai UniversityRequires access

Structure of Neutral Polysaccharides from Skin of Aloe vera Irrigated with Seawater

Changhong Wang

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Abstract

After being extracted with hot water and precipitated with alcohol, crude polysaccharide is obtained from skin of Aloe vera. 4 Fractions are prepared by further purification with cellulose DEAE-52. SAPS-A is a most fraction which is made of neutral monosaccharides. From fraction SAPS-A, a purer fraction SAPS-1 is separated by sephadex G-100. The analysis of sepharose 4B, UV, IR and NMR show SAPS-1 is a 6-acetylated partially β-(1-4)-D-Mannan, whose weight is 40±2 kDa.

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After being extracted with hot water and precipitated with alcohol, crude polysaccharide is obtained from skin of Aloe vera. 4 Fractions are prepared by further purification with cellulose DEAE-52. SAPS-A is a most fraction which is made of neutral monosaccharides. From fraction SAPS-A, a purer fraction SAPS-1 is separated by sephadex G-100. The analysis of sepharose 4B, UV, IR and NMR show SAPS-1 is a 6-acetylated partially β-(1-4)-D-Mannan, whose weight is 40±2 kDa.

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Available abstract

After being extracted with hot water and precipitated with alcohol, crude polysaccharide is obtained from skin of Aloe vera. 4 Fractions are prepared by further purification with cellulose DEAE-52. SAPS-A is a most fraction which is made of neutral monosaccharides. From fraction SAPS-A, a purer fraction SAPS-1 is separated by sephadex G-100. The analysis of sepharose 4B, UV, IR and NMR show SAPS-1 is a 6-acetylated partially β-(1-4)-D-Mannan, whose weight is 40±2 kDa.

Key concepts: Polysaccharide, Aloe vera, Chemistry, Sephadex, Fraction (chemistry), Monosaccharide, Chromatography, Seawater

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