Some biological characters in the multidrug resistant sub-cell line of hepG2

Objective In order to study the multidrug resistance mechanism in primary hepatic carcinoma,to establish a multidrug resistant hepatocellular carcinoma cell line (HepG2/ADM) and investigate its biological behaviors.Methods Through exposure to gradually increased concentration of ADM,the cell line HepG2 was induced to form a multidrug resistant subcell line (HepG2/ADM).Western-blot-enhanced chemilluminesence (ECL) was used to detect the expression products of MDR1,MRP and LRP in the two cell lines.Results The doubling time of HepG2/ADM was 2.05 times longer than HepG2.The expression of p-170 in HepG2/ADM was 3.9 times more than its parent cell line.But there was no significant difference in the expressions of MRP and LRP between the two cell lines.Conclusion Compared with its parent cell line,the doubling time and multidrug resistance of HepG2/ADM had changed greatly,and its multidrug resistance was mainly correlated with a higher expression of MDR1.