Study on Tuber Plant Esterase in Detection of Organophosphorus Pesticide Residues

Through the application of the enzyme inhibition mechanism to the enzymatic analysis, five tuber plants(Solanum tuberosum, Ipomoea batatasM, Sagittaria sagittifolia, Colocasia esculenta(L.) Schoot, Nelumbo nucifera Gaertn) were used to select botanical esterase suitable for the detection of pesticide residues by comparing the general and specific activities of the phytoesterase. The results showed that the general esterase activities in tuber plants Solanum tuberosum, Sagittaria sagittifolia and Nelumbo nucifera Gaertn were 8.4623, 8.7437, and 7.4910, respectively, and the optimal operation condition were searched in the detection. The sensitivity and the limit of detection(LOD) of phytoesterase to organophosphate pesticides were also studied. The results showed that 3 types of phytoesterase were not only very sensitive to organophosphate pesticides but also their LOD were much less than the maximum residue limits(MRL) of the provision of the nation specified. So, we concluded that phytoesterase obtained from 3 tuber plants could be used for the rapid detection of pesticide residues.