2008•Journal of Shanxi Medical College for Continuing EducationRequires access
Effects of Propofol and Ischemic Preconditioning on Cell Apoptosis Induced by Hepatic Ischemia-Reperfusion Injury in Rats
Chunyan Yang
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Abstract
Objective:To investigate the effects of propofol and ischemic preconditioning on cell apoptosis induced by hepatic ischemia-reperfusion injury in rats.Methods: Seventy-two adult healthy SD rats were randomly divided into 4 groups(n=18).They were a sham-operated group(Sham group),an ischemia-reperfusion group(IR group),an ischemic preconditioning group(IP group)and a propofol group(P group).A 70% hepatic ischemia-reperfusion model was made and the left hepatic lobes were sampled after the experiment.The expression of Bcl-2 and Bax protein in liver tissue was assessed by immunohistochemical method and the apoptosis index(AI)of hepatic cells was measured by TUNEL method.Results: Compared with the Sham group,the expression of Bcl-2,Bax protein and the apoptosis index of hepatic cells in each group were significantly increased(P0.05).Compared with the IR group,the expression of Bcl-2 protein in the IP group and the P group was significantly increased while the expression of Bax protein and AI significantly decreased(P 0.05).Compared with the IP group,the expression of Bcl-2 protein in the P group was increased while the expression of Bax protein and AI decreased(P0.05).Conclusion: Propofol and ischemic preconditioning can relieve the liver cellular apoptosis by regulating the expression of Bcl-2 and Bax protein and may protect liver from ischemia-reperfusion injury in rats.