Study on Enzymatic Hydrolysis of Whey Protein Process

We chose whey protein as the raw material and selected alkaline protease(Alcalase) to hydrolyze whey protein.Through the four factors and three levels orthogonal experimental designed method(enzyme and substrate ratio(E/S),pH values,hydrolysis temperature,hydrolysis time) to optimize whey protein hydrolysis process conditions.Determined the alkaline protease(Alcalase) enzymatic hydrolysis of whey protein optimum conditions were enzyme and substrate concentration ratio 0.05,pH 8.0,reaction temperature 60 ℃,hydrolysis 200 min.Under these conditions,the degree of hydrolysis was 21.92 %.These factors influenced the degree of hydrolysis in the order enzyme substrate concentration ratio(E/S)hydrolysis temperaturehydrolysis timepH value.