Research of new progress to preparation maize resistant starch

High-amylose maize starch were used as raw material in this study. The combination of autoclaving-cooling(121 ℃ 20 min then 4 ℃ 24 h) and acid hydrolysis was used to prepare resistant starch from them. The treatment times of autoclave heating and cooling, together with types, concentration and hydrolysis time of acids were studied and compared. The results showed that the yield of crude resistant starch from both maize starches was significantly affected by factors studied. And the optimum condition to preparation resistant starch with this method was: after twice of autoclaving-cooling, added citric acid until the concentration was 0.1 mol/L, then hydrolyzed for 12 h at room temperature. The yield of resistant starch with such process would be 39%. The effect of acid hydrolysis on high-amylose maize starch was significant for resistant starch preparation.