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Research on Real-Time Quantitative RT-PCR Detection Method of MGF-mRNA

LU Yao-fei

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Abstract

Objective;Establish the real-time quantitative RT-PCR method for detecting the gene expression of mechano growth factor(MGF).Methods:The MGF gene expression in gastrocnemius was detected by the real-time quantitative RT-PCR method.Results;The MGF mRNA expression in gastrocnemius could be absolutely measured by the real-time quantitative RT-PCR method with the correlation coefficient(r0.99).

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What this paper is about

Objective;Establish the real-time quantitative RT-PCR method for detecting the gene expression of mechano growth factor(MGF).Methods:The MGF gene expression in gastrocnemius was detected by the real-time quantitative RT-PCR method.Results;The MGF mRNA expression in gastrocnemius could be absolutely measured by the real-time quantitative RT-PCR method with the correlation coefficient(r0.99).

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Available abstract

Objective;Establish the real-time quantitative RT-PCR method for detecting the gene expression of mechano growth factor(MGF).Methods:The MGF gene expression in gastrocnemius was detected by the real-time quantitative RT-PCR method.Results;The MGF mRNA expression in gastrocnemius could be absolutely measured by the real-time quantitative RT-PCR method with the correlation coefficient(r0.99).

Key concepts: Real-time polymerase chain reaction, Messenger RNA, Quantitative analysis (chemistry), Gene expression, Molecular biology, Quantitative assessment, microRNA, Gene

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