2004•Unpublished venueRequires access

Stable expression of imidazoline-1 receptor in mammalian cells

Lu Xin

Abstract

Objective: To stably transfect imidazoline-1 receptor (I 1R) in Chinese hamster ovary (CHO) cells. Methods: I 1R Protein cDNA was stably transfected in CHO cells by the method of lipofectamine. Results: The cell line that stably expressed I 1R without the expression of α 2-adrenergic receptors was constructed. This cell line provided a perfect cell model to investigate the function and signal transduction pathway of I 1R. Conclusion: A cell line with stable expression of I 1R was constructed.

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Key concepts: Chinese hamster ovary cell, Transfection, Receptor, Lipofectamine, Imidazoline receptor, Cell culture, Complementary DNA, Chinese hamster