A pandemic group-specific multiplex PCR assay application in identification of pandemic vibrio parahaemolyticus

Objective In order to set up rapid identification for the pandemic vibrio parahaemolyticus,develope a pandemic group-specific multiplex PCR assay for base unit. Methods To find out the sequence of vibrio parahaemolyticus,design a multiplex PCR primers for pandemic vibrio parahaemolyticus amplification. Results The aim gene fragment can be amplified by a multiplex PCR assay,it largely reduce the time of identification.Conclusion The pandemic group-specific multiplex PCR assay can fast identify pandemic vibrio parahaemolyticus and providing a methodology for searching epidemic pathogen.