ScholarLens Back to search
2008•Unpublished venueRequires access

RAPD and SCAR Markers Linked to Fulvia fulva Resistant Gene Cf6 in Tomato

Fenglan Huang

Open publisher page 0 citations

Abstract

【Objective】The study identified the molecular marker linked to Fulvia fulva resistant gene Cf6 in tomato. 【Method】 With a F2 population between a resistant and a susceptible parent (03036×03748), the linked marker was screened by BSA method and RAPD technology. 【Result】 Two specific bands were found at the 500 bp region using F2-based bulked segregant analysis plants. An RAPD marker was tightly linked to Fulvia fulva resistant gene Cf6 in tomato, with a genetic distance of 8.7 cm, The RAPD marker was converted into SCAR marker. Sequencing of the fragment indicated that the length was 619 bp and 559 bp, respectively.【Conclusion】SCAR markers could be applied to identify the resistance to Fulvia fulva, and can be used in molecular marker-assisted breeding practice.

About this research paper

What this paper is about

【Objective】The study identified the molecular marker linked to Fulvia fulva resistant gene Cf6 in tomato. 【Method】 With a F2 population between a resistant and a susceptible parent (03036×03748), the linked marker was screened by BSA method and RAPD technology. 【Result】 Two specific bands were found at the 500 bp region using F2-based bulked segregant analysis plants. An RAPD marker was tightly linked to Fulvia fulva resistant gene Cf6 in tomato, with a genetic distance of 8.7 cm, The RAPD marker was converted into SCAR marker. Sequencing of the fragment indicated that the length was 619 bp and 559 bp, respectively.【Conclusion】SCAR markers could be applied to identify the resistance to Fulvia fulva, and can be used in molecular marker-assisted breeding practice.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

【Objective】The study identified the molecular marker linked to Fulvia fulva resistant gene Cf6 in tomato. 【Method】 With a F2 population between a resistant and a susceptible parent (03036×03748), the linked marker was screened by BSA method and RAPD technology. 【Result】 Two specific bands were found at the 500 bp region using F2-based bulked segregant analysis plants. An RAPD marker was tightly linked to Fulvia fulva resistant gene Cf6 in tomato, with a genetic distance of 8.7 cm, The RAPD marker was converted into SCAR marker. Sequencing of the fragment indicated that the length was 619 bp and 559 bp, respectively.【Conclusion】SCAR markers could be applied to identify the resistance to Fulvia fulva, and can be used in molecular marker-assisted breeding practice.

Key concepts: RAPD, Bulked segregant analysis, Biology, Molecular marker, Genetic marker, Genetics, Gene, Population

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
RAPD and SCAR Markers Linked to Fulvia fulva Resistant Gene Cf6 in Tomato — Research Paper | ScholarLens