Progress of application and study of FRET technology in life science

Fluorescence resonance energy transfer(FRET) is the energy transfer phenomena between two fluorophores by dipole-dipole coupling effect through non-radiative pathways.It can be used to measure the distance between molecules.FRET probes include fluorescent proteins,organic fluorescent dyes,lanthanide dyes and quantum dot,they have had many new applications recently.Combined with some new technologys such as time-resolution,single molecule detection,fluorescence lifetime-resolved imaging and fluorescence correlation spectroscopy,FRET gets more efficient.