Temporospatial expression of zif268mRNA and Zif268 protein in hippocampus in rats after kindling of temporal lobe epilepsy

Objective To explore the relationship of the temporospatial expression of zif268mRNA and its protein in hippocampus with the epileptogenous brain injury in temporal lobe epilepsy(TLE).Methods Male wistar rats were randomly divided into three groups:six were in normal group,36 rats were in each of the other two groups,sham group and kainic acid(KA) kindled TLE group(TLE group),and were subdivided into 6 subgroups according to the time points of 6h,24h,3d,7d,14d,21d after KA kindling,respectively.Each subgroup had 6 rats.Typical temporal lobe epileptic model was established by microinjected KA into right nucleus amygdalae in rats.In situ hybridization and immunohistochemistry method were used to spatio-temporally observe the expression of zif268mRNA and their protein in hippocampus in rats after kindling or sham operating,respectively.Results In general or 21d after KA kindling,zif268mRNA were highly expressed in CA1,CA3 and DG regions in hippocampus in TLE group than in that of sham group(P0.05).On the whole,the expression of Zif268 protein was significantly decreased than that of sham group(P0.05),and it was same on 21d after KA kindling(P0.05).Regression analysis showed that the expression of zif268mRNA in hippocampus was positively correlated with TLE epileptogenesis(β= 0.286,P0.001),while the Zif268 protein was negatively correlated(β=-0.153,P0.001).Conclusion The spatio-temporal changes of the expression of zif268mRNA and Zif268 protein may be involved in the mechanisms underlying TLE and subsequent brain injury.