Determination of inorganic arsenic in seaweeds with HPLC-HG-AFS

This paper is about the research of the determination of inorganic arsenic in edible seaweeds with High performance liquid chromatography-hydride generation-atomic fluorescence spectrometry(HPLC-HG-AFS).The curved surface fitting method was applied to establish optimum conditions of extraction reagent,time and temperature based on the inorganic arsenic contents of Sargassum fusiforme.It was found that the optimum conditions is with 0.1% HCL at 52℃ for 78 min.The detection limit of methodology was 0.0 628 mg/kg.The recovery rates of 1.0 mg/kg and 10.0 mg/kg in samples were between 80.5% and 105.7%,and the relative standard deviations were between 4.5% and 5.0%.The content of inorganic arsenic of sixty types of laver from different areas in Fujian Province was examined.The results showed that inorganic arsenic concentration in laver was below,which accounted for the total arsenic concentration of 0 ~ 1.96%.