Construction of The pcDNA3.1(+)-MS Eukaryotic Expression Plasmid

Objective To construct and identify the pcDNA3.1(+)-MS eukaryotic expression plasmid. Methods Extracted DNA from M. Tuberculosis was amplified by PCR and the target gene we got was cloned into the unique HindⅢ and EcoRⅠcloning sites of pcDNA3.1(+). Results The accuracy of pcDNA3.1(+)-MS plasmid constructs was confirmed by a series of molecularbiology techniques. Conclusion The construction of pcDNA3.1(+)-MS provided the possibility for investigating immunogenicity of the recombinant plasmid and studying on the role of the signal peptide in the protein expression and excretion, and preparation of a new tuberculosis vaccine.