Optimization of Fermentation Medium Components for Natamycin Producing by Streptomyces lydicus G117

In order to increase the yield of natamycin produced by Streptomyces lydicus G117,fermentation medium was optimized with methods of Plackett-Burman,path of steepest ascent and Box-Behnken Design. PlacketBurman was applied to screen three main factors that affecting production of natamycin,including corn starch,soybean powder and Na Cl. The result indicated that the optimum mass concentrations of the three components in fermentation medium were determined to be 46. 15,14. 80,5. 88 g / L,respectively,with the highest theoretical prediction yield of 2 708. 06 mg / L. In subsequent 3 fermentation experiments,the average yield of natamycin for the optimized fermentation medium was 2 718. 57 mg / L,which there was no significant difference with the theoretical prediction yield,and increased by 33. 2% in comparison with basic fermentation medium.