Co-culture of Bacillus subtilis and Bacillus thuringiensis and its influence on production of bioactive substances

The bacterial growth,production of antimicrobial lipopeptides and toxic parasporal crystal protein were studied under the condition of combination of Bacillus subtilis Bs55 and Bs90 strains with Bacillus thuringiensis Bt1 and Bt2 strains.The results showed that the bacterial growth and the production of lipopeptide in combination of Bs55 with Bt strains were similar to Bs55 alone and parasporal crystal protein was not detected but Bt characteristic protein was found.While bacterial growth and the lipopeptide production in combination of Bs90 with Bt strains were relatively different from Bs90,and crystal protein was detected.The results indicate that Bacillus subtilis and Bacillus thuringiensis can be co grown,and can produce their bioactive substances in a suitable combination.