Application Research on Preparation of High Nucleic Acid Yeast Extract Using Waste Beer Yeast

This article is specifically for the increasing demands of domestic and foreign markets for extracts that are rich in I+G to develop beer yeast extract of high nucleic acid type.Adopt step-bystep enzymatic hydrolysis of beer yeast for making high nucleic acid yeast extract,and obtain the optimal enzymatic hydrolysis process conditions.The optimal conditions for yeast extraction enzyme enzymolysis are:substrate concentration of 10%,adding amount of extraction enzyme of yeast suspension of 0.04%,enzymolysis time of 22 hat 55℃,pH value of 6.5.The optimal conditions for nucleic acid enzyme enzymolysis are:substrate concentration of 10%,adding amount of nucleic acid enzyme of yeast suspension of 0.02%,enzymolysis time of 6hat 70 ℃,pH value of 5.0.Finally,the flavor nucleotides content of yeast extract(I+G)increases from 3%～4%to 10%～12%.