Influence of autophagic apoptosis induced by vincristine on mitochondrial transmembrane potentials

Purpose: To understand if vincristine (VCR) is related to the alteration of mitochondrial transmembrane potentials (A) and its possible mechanisms. Methods: Human liver cell line L-02 was used as in vitro models. The was detected by the double staining of propidium iodide (PI) and Rhodamine 123(Rhl23),which fluorescent intensities were measured by flow cytometry, while apoptosis was confirmed by sub-G1 cell content as well as transmission electron microscopy observation. Results and Conclusion: In the cells sensitive to VCR-induced autophagic apoptosis, the was disrupted with VCR treatment. Vincristine can induced L-02 cell autophagic apoptosis, which could associate closely with the collapse. The disruption of the maybe is the key event of VCR-induced autophagic apoptosis.