2010•Chinese Journal of Gastroenterology and HepatologyRequires access
Changes of the apoptosis-related gene in Fas-expressed LoVo colorectal cancer cells
Bing Xiao
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Abstract
Objective To observe the change of the apoptosis-related gene in Fas-expressed colon cancer cell,and to discuss the transmitting approach of apoptosis signal. Methods The expression of apoptosis gene in Fas-expressed colon cancer cells was detected by FICM.Cells were divided into Fas-transfected cells (control),Fas-transfected cells + Fas antibody(12 h),Fas-transfected cells + Fas antibody(24 h). Results The expression of bcl-2 declined significantly(P0.01) and the expression of bax expression increased(P0.05) in Fas-transfected cells with Fas antibody.The change of the expression of apoptosis gene under Fas inducing apoptosis was validated partially. Conclusion The apoptosis is effectively induced by Fas antibody in Fas-expressed colon cancer cells,with the bcl-2 down-expressed and the bax up-expressed inducing apoptosis.