2007•He'nan nongye daxue xuebaoRequires access
Transformation and Expression of Nicotiana tobaccum L.with a Gene Encoding for the Betaine Aldehyde Dehydrogenade(BADH) from Triticum aestivum L.
Qi Zou
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Abstract
Betaine aldehyde dehydrogenase gene(BADH) cDNA cloned from Triticum aestivum L.in the plasmid pBIH containing CMV 35S promoter and NPTII gene was transffered into tobacco(Nicotia-na tobaccum L.) by Agrobacterium tumefaciens-mediate with 80% of average frequency of transformation.Transgenic plants were obtained showing BADH integration by both PCR and PCR-Southerh blot.There were great differences in BADH activity between transgenic plants after low temperature treatment,some transgenic plants had higer BADH activity of 4.8 nmol·mg-1 protein·min-1 but others had none.