Expression of 19-kDa Protein of Mycobacterium Tuberculosis in Escherchia Coli

Objective To obtain recombinant 19-kDa protein from Mycobacterium tuberculosis by using gene engineering technology.Methods The gene coding 19-kDa protein inserted into a expression vector pET24b,and then transferred E.coli BL21(DE3).The expressed product was identified by SDS-PAGE,Plasmid pET24b containing19-kDa gene was transferred into competent Escherichia coli BL21(DE3) and 19-kDa gene was over expressed by the inducement of IPTG.Results The recombinant 19-kDa protein was expressed in soluble form in E.coli. When the culture was induced for 3～4 hours,the recombinant 19-KDa protein was produced in highest quantity .Conclusion Escherichia coli containing recombinant plasmid pET24b-19-kDa gene could express recombinant 19-KDa protein in high level.